Transfection

PTG1 is a cationic polymer that provides highly efficient, low-toxic DNA transfections from different types of mammalian cells.
PTG1-plus is a BPF certified PTG1 derivative that is very efficient for the bioproduction of recombinant proteins and viruses obtained from transient transfection of HEK293 and CHO-S cells.

PTG1 forms complexes with nucleic acids which can be encapsulated in liposomes to form nanoparticles called Lipopolyplexes or LPRs which increase transfection efficiency.


LPRs allow good in vivo transfections of mRNA and microRNA.

Simion et al., Intracellular trafficking and functional monitoring of miRNA delivery in glioblastoma using lipopolyplexes and the miRNA-ON RILES reporter system. J. Controlled Release 2020, 327:429-443.

Wang et al., In Vivo bone tissue induction by freeze-dried collagen-nanohydroxyapatite matrix loaded with BMP2/NS1 mRNAs lipopolyplexes. J.Controlled Release 2021; 334; 188–200.